Mothur
用于微生物生态学分析的综合工具，专注于 16S rRNA 基因序列分析和微生物群落多样性研究
基本信息
- 类别: amplicon-analysis
- 版本: 1.48.5
- 难度: intermediate
- 最后更新: 2026-03-18
链接
安装
bash
# Ubuntu/Debian
sudo apt-get install mothur
# Conda
conda install -c bioconda mothur
# 源码编译
git clone https://github.com/mothur/mothur.git
cd mothur
make -j使用示例
标准 MiSeq SOP
mothur > make.contigs(file=stability.files, processors=8) mothur > screen.seqs(fasta=current, maxambig=0, maxlength=275) mothur > unique.seqs(fasta=current) mothur > align.seqs(fasta=current, reference=silva.v4.fasta) mothur > filter.seqs(fasta=current, vertical=T, trump=.) mothur > pre.cluster(fasta=current, diffs=2) mothur > chimera.vsearch(fasta=current) mothur > classify.seqs(fasta=current, reference=trainset9_032012.pds.fasta) mothur > cluster.split(fasta=current, count=current, splitmethod=classify)
标签
16S amplicon OTU diversity SOP